House of 1000 Corpses received a generally negative critical reception upon its release. Frank Scheck of The Hollywood Reporter wrote that the film "lives up to the spirit but not the quality of its inspirations" and is ultimately a "cheesy and ultragory exploitation horror flick" and "strangely devoid of thrills, shocks or horror."[37] Clint Morris of Film Threat slammed the film as "an hour and a half of undecipherable plot" and found the film to be "sickening" overall.[38] James Brundage of Filmcritic.com wrote that the film was simply "hick after hick, cheap scary image after cheap scary image, lots of southern accents and psychotic murders," and was "too highbrow to be a good cheap horror movie, too lowbrow to be satire, and too boring to bear the value of the ticket."[39] Slant Magazine gave the film two out of four stars, stating "If not for the blink-and-miss sideshow attractions and stockpile of memorable quotes, [House of] 1000 Corpses would have been easier to shrug off. This vintage curio is proudly and humorously derivative but that familiar aftertaste is that of wasted opportunities."[40] The New York Times also had a negative review of the film, writing "As much as film buffs might enjoy recognizing references to Motel Hell and other drive-in classics, Mr. Zombie's encyclopedic approach to the genre results in a crowded, frenzied film in which no single idea is developed to a satisfying payoff."[41] The review also criticized the cutaway scenes and home footage used throughout the film, adding "Mr. Zombie is both too much of a stylist, always cutting away to oddball inserts, black-and-white flashbacks, negative images and much else, and too little: he is not in enough control of his means to let a mood grow and fester. And festering is what this kind of film is all about."[41]
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Cancer has a significant impact on public health worldwide. One strategy to lower its burden is through cancer screening and early diagnosis. It is well known that patients have a higher cure rate and 5-year survival if diagnosed at early stages [1]. Medical expense increases dramatically with the stage [2, 3]. Tissue biopsy is the most widely-used tool for cancer detection, staging, and prognosis, but sometimes tumor tissue can be difficult to obtain, especially in metastatic diseases like late-stage lung cancer. Moreover, it is unrealistic to use tissue biopsy for cancer screening and early diagnosis when the tumors have not formed yet. Currently, there are some screening methods proved to be useful for cancer prevention. For example, the mammogram is the best way to detect breast cancer; Pap test is used for early detection of cervical cancer; regular colorectal cancer screening and low-dose computed tomography are recommended to reducing mortality from colorectal cancer and lung cancer, respectively [4]. However, all of these screening methods have limited sensitivity and specificity and are only applicable to a unique cancer type. In order to perform large-scale cancer screening among healthy individuals in the future, a more general and cost-effective approach is needed. In recent years, many scientists and companies have cast their eyes on liquid biopsy [5,6,7,8]. Blood contains many types of biological materials like circulating cells, platelets, extracellular vesicles, mRNA, miRNA, protein, and cell-free DNA (cfDNA) [9]. From the blood of cancer patients, a portion of the cfDNA is released by tumor cells through apoptosis, necrosis, or active release [10], and this DNA is called circulating tumor DNA (ctDNA). The tumor-specific mutations in ctDNA sequence can act as a new type of cancer biomarker and help to identify cancer patients from a group of healthy individuals. Compared to traditional cancer diagnosis using tissue biopsy, liquid biopsy is more feasible and less invasive and is more comprehensive than tissue biopsy to evaluate tumor heterogeneity [11] because all tumor sites will release ctDNA into the blood. Facilitated by the rapid development of next-generation sequencing (NGS) technologies, nowadays, ctDNA sequencing can achieve much higher sensitivity than tissue biopsy and can be designed for different purposes [12].
"O, Adam," cried Gaston with sound 'twixt laugh and choke, "brave youth,gentle boy, dost boggle and trouble thyself for such vile dog? Know thenhe was dead as the meat so salt we devour ... I shot him 'twixt theeyes, so think no more of thing so small. But for thyself, Adam, thyvalour is to my cowardice the rebuke ... and thou so young I might bethy father! But, verily, this horror of water makes of me the child....Hark thee, Adam ... years since I have a friend but we quarrel, wefight, I ... kill him and am the desolate one always ... and so now I amchoke in this terrible sea ... it is just. And now, go comfort thy lady,it is, mayhap, that she needs thee more even than I ... go, my Adam!"
So lay I some while until, my strength recovered somewhat, I struggledto my knees and turned. But now, beholding what horror of sea I had comethrough, I hid my eyes appalled and crouched thus on my knees, gaspedforth my thankfulness to that most merciful God that He should havewrought me such miracle.
I opened my eyes to the comfort of a fire that blazed and crackled rightcheerily, putting me to such surprise that I would have sat up thebetter to look about me but found movement so painful that I sank backgroaning, and in this moment came Barbara to kneel beside me.
"Blood!... Oh, Adam ... was it you? Ah, what have you done? God forgiveyou ... there is a father's blood betwixt us ... always and forever.... Oh God, pity!" And as she looked up at me I read in her wideeyes bitter reproach and horror of me. Then was clamour on the stair,clattering feet, shouts and uproar.... Sword advanced, I turned to meetwhat might be, but she barred my way arms out-flung against me, in hereyes that same stark horror. And so, scarce knowing what I did, I leaptto the open casement and, forcing my way through, plunged out and down.
"... he is known, my lord, as Adam Thursday, calling himself LordBellcastonborough. He is also known to have been consorter withproscribed rebels themselves in frequent communication with the accursedPretender Stuart himself, a go-between, my lord, a bearer of letterstreasonable, a complotter against the King's most excellent Majesty andthe weal of the realm." Here the aged, magnificent gentleman closed hisvital eyes so that his face seemed instantly dead, and spoke with softand furry indistinctness:
"Yes, I did write it," she cried, pouring out her soul in a torrent of words. "I did write it. Why should I deny it? I have no reason to be ashamed of it. I wished him to help me. I believed that if I had an interview I could gain his help, so I asked him to meet me."
The view from them was confused, nor did any fresh object meetour observation. We had, however, considerably neared the twowooded hills, and the elevations that from the range were to theS.W., now bore N.W. of us. We had wandered too far from the camp toadmit of our returning to it to sleep; we therefore commenced asearch for water, and having found some, we tethered our horsesnear it for the night, and should have been tolerably comfortable,had not the mosquitoes been so extremely troublesome. They defiedthe power of smoke, and annoyed me so much, that, hot as it was, Irolled myself in my boat cloak, and perspired in consequence to such adegree, that my clothes were wet through, and I had to stand at thefire in the morning to dry them. Mr. Hume, who could not bear suchconfinement, suffered the penalty, and was most unmercifullybitten.
New Year's Range consists of a principal group of five hills,the loftiest of which does not measure 300 feet in height. It haslateral ridges, extending to the N.N.W. on the one hand, andbending in to the creek on the other. The former have a fewcypresses, sterculia, and iron bark upon them; the latter aregenerally covered with brush, under box; the brush for the mostpart consisting of two distinct species of stenochylus, and a newacacia. The whole range is of quartz formation, small fragments ofwhich are profusely scattered over the ridges, and are abundantlyincrusted with oxide of iron. The soil in the neighbourhood of NewYear's Range is a red loam, with a slight mixture of sand. An openforest country lies between it and the creek, and it is not at alldeficient in pasture.
We passed some considerable plains, lying to the eastward of thecreek, on parts of which the grass, though growing in tufts, was ofluxuriant growth. They were, however, more generally covered withsalsola and rhagodia, and totally destitute of other vegetation,the soil upon them being a red sandy loam. The paths across theplains, which varied in breadth from three to eight miles, werenumerous; but they had not been recently trodden. The creekcontinued to have a thick brush of casuarina and acacia near it, tothe westward of which there was a rising open forest track; thetimber upon it being chiefly box, cypress, and the acacialongifolia. It was most probably connected with New Year's Range,those elevations being about thirty miles distant. It terminated insome gentle hills which, though covered in places with acaciashrub, were sufficiently open to afford an extensive view. Fromtheir summit Oxley's Table Land, towards which we had beengradually working our way, was distinctly visible, distant abouttwenty miles, and bearing by compass W. by S. On descending from these hills*which were scattered over with fragments of slaty quartz, wetraversed a box flat, apparently subject to overflow, having abarren sandy scrub to its left. I had desired the men to preserve aW.N.W. direction, on leaving them, supposing that that course wouldhave kept them near the creek; but, on overtaking the party, Ifound that they had wandered completely away from it. The fact was,that the creek had taken a sudden bend to the eastward of N. andhad thus thrown them out. It was with some difficulty that weregained it before sunset; and we were at length obliged to stopfor the night at a small plain, about a quarter of a mile short ofit, but we had the satisfaction of having excellent feed for theanimals. 2ff7e9595c
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